Eco-Friendly Microbes for the Environmental Cleanup

Karachi is the largest city in Pakistan and has a population of 20 million and an area of 3780 km2. Karachi produces more than 9,000 tons of municipal waste daily. Nearly 400 million gallons of untreated wastewater are disposed of in the Arabian Sea on a daily basis. This wastewater is laden with plastics, which comprise 9% of the total waste, heavy metals, while 30% of it is food waste which comprises mainly of fats, oil, grease (FOG), proteins, and carbohydrates. 

Plastic-strewn drains lead to a clogged drainage system which causes them to overflow every rainy season flooding the city with the sewage water. Single-use plastic, particularly, proves to be a greater nuisance as it is the majority that ends up in landfills and drains.

Even waste holds a lot of potentials which is unfortunately not fully realized. This waste possibly harbors bacteria; producing enzymes that can degrade plastic, carbohydrates, proteins, or lipids. Isolating such bacteria and adapting them to high concentrations of one or more of the above molecules can significantly improve the waste crisis at hand.

Plastics dumped in landfills react with water and form hazardous chemicals. These landfills harbor microorganisms that have adapted to the environment strewn with different types of plastics. The microorganisms include bacteria such as Pseudomonas, nylon-eating bacteria, and Flavobacteria. These bacteria break down nylon through the activity of the nylonase enzyme

Bioremediation is a branch of biotechnology that needs to be actively used given the current circumstances of pollution in the environment. Wastewaters already harbor microbial species that possess the genes to allow them to use such polymers as energy sources. They can be adapted to improve their bioremediation efficiency under lab conditions and then use commercially to speed up the processes of biodegradation. 

Rapid industrialization and urbanization have led to the release of vast quantities of waste pollutants (including new toxins) into the environment, which in turn has led to an increase in pollution. The bulk of colored effluents from the textile dyestuff and dyeing industries, which contain dyes, are being released into the environment at an increasing rate. Azo dyes make up more than half of the weight used for coloring purposes and are the most widely used colorants in the textile sector. These chemicals have the ability to increase the color of azo compounds and provide bonding affinity groups. Azo dye-containing effluents are poisonous, phytotoxic, carcinogenic, and mutagenic in addition to being recalcitrant. The general environment dictates the necessity of treating dye-containing water before disposal.

Numerous physicochemical decolorization approaches have been utilized during the past 20 years, but only a select few have found favor with the textile industry. The ongoing use of these techniques has led to drawbacks like high costs, the production of sludge, and the release of harmful compounds. In addition, several of these techniques don't totally get rid of the organic compounds that are the root of secondary contamination. Under certain environmental conditions, microorganisms can totally mineralize synthetic colors or degrade them to non-colored chemicals. The microbial decolorization of dyes has frequently been seen as an economically viable solution with the potential for technical development due to its affordability and ecological compatibility.

Microbes for the Environmental Cleanup

Research Project for Sindh HEC Research and Technology Showcase 2022


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